The centrifuge distributes the blood components, depending on their specific bearings, that is, the CFIs are the heaviest, followed by WBCs, although platelets are the lightest. The first centrifugation is slow, so that platelet spinning is avoided and plasma isolation occurs easily. Platelets are mainly concentrated on the top of the buffy coat series. Subsequent centrifugation is faster, so that platelets occur down, leaving the bottom platelet (PPP) plaster (PPP) above to separate as a pad at the bottom of the tube. The final concentration of platelets depends on the volume reduction of the PPP. About three-quarters of the floatable is set aside, and the platelet-rich pellet is restored in the rest of the plasma. The suspension is used as PRP. The double turning method is preferable to the single twist method, as the use of the latter does not achieve the therapeutic concentration of platelets.
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The total blood is collected by whistleblowing in acid citrate (ACD) tubes.
The cold is not cooled at any time before or during the separation of platelets.
There is a centralized mode using a ‘soft’ twist.
Plasma platelets are added to another sterile tube (without anti-coagulant).
Edit the second tube at a higher speed (hard turn) to obtain platelet concentration.
PRP is the third lowest, and two-thirds are called plasma plaques. Platelet pellets are made at the bottom of the tube.
PPP is removed, and platelet pellets are suspended in a minimum plasma quantity (2-4 ml) by gently shaking the tube.
Buffy coat method
The total blood is stored at 20-24 ° C before centrifugation.
After storage, the blood is centrifuged at ‘high’.
The density is made up of three layers: The bottom layer is RBCs, the middle layer is platelets and WBCs and the PPP layer is the top.
Plassema is added to the top of the container.
The buffy coat layer is transferred to another sterile tube.
Various automated devices and equipment are available in the market.
We use Bio-equipment and centrifuge to prepare PRP.2 Vials, and 13.5 whole blood blends with each of 1.5 ml of ACD-A solution and centrifuged for 4 minutes at 3000 RPM. The PRP removes 5–6 ml of buffy coat from these two vials and is injected as required. It gives a concentration of five to seven hours of base platelet count.
They proposed a classification in 2009 whereby platelet concentrations can be classified into four main households depending on their cell content and fibrin architecture.
Pure plasma (P-PRP) or platelet-rich preparations containing poor platelets and poor leucocyte and having a low density fibrin network on activation. They can be used as liquid solutions or as activation gel. Therefore, it can be injected (as used in sports medicine) it or it can be during gelling on wound or on seam (such as fibrin glue). After the first slow centrifugation of twisting, only the majestic surface coat layer is adsorbed and taken for the second centrifugation. Example: PRGF (plasma is rich in growth factors or preparations that are rich in growth).
Prepare full-rich plasma products and platelets-L-PRP with leococytes and low density fibrin network formation on activation. This group owns the largest number of commercial or experimental systems. During the preparation of L-PRP, PPP, a full and upper buffy coat layer 1-2 mm of a red blood layer is piped after the first centrifugation. Like P-PRP, they can be in liquid form or gel.
Fiber-rich pipes (P-PRF) or plate-rich platelet preparations are rich in platelets, which are poor leucocyte and have a high density fibrin network. They are only in the form of strongly activated formulation. To perform P-PRF, P-PRP is mixed with an agent and a specific gel is used. After its incubation for some time, a stable fiber matrix (PRFM) is created. It cannot be injected or used such as traditional fiber glue but because it has a strong fiber matrix, it can be treated as a very solid material and used for a variety of other applications.
Fibrin (L-PRF) full-fuel platelets or second generation PRP product preparations with leococytes and high density fibrin network. The blood is centrifuged immediately after collection without anti-coagulant, thrombin or CaCl2. Natural coagulation process leads to three layers – the lowest RBC layer, L-PRF medium layer and plasma layer best. The PRF coagulation is pressed between two gauze to create a strong membrane. Like P-PRF, it is in the form of a strongly activated formulation.
Fiber leukocyte is platelet rich fibrin is any material with fiber-rich platelets and platelet function as binding sites for platelets and growth factors. Three local concentrations of growth factors to increase a specific tissue, PRF offers advanced tissue regeneration.
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